Last data update: 24 January 2024 16:39 CET
Host name: Serratia fonticola GFP (LMBP 13927)
| New search | Print data sheet |
| Species: | Serratia fonticola |
| Strain designation: | GFP |
| Accession number: | LMBP 13927 |
| Depositor: | This host strain was deposited by F. Waegenaar1 and Prof. Dr N. Boon1. 1 Center for Microbial Ecology and Technology CMET, Faculty of Bioscience Engineering, Ghent University, Belgium |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox + ampicillin (50 µg/ml) + tetracyclin (10 µg/ml) |
| Selection marker: | Ampicillin, tetracyclin |
| Temperature: | 28°C |
| Genotype: | - |
| Phenotype: | Serratia fonticola (TaxonID 47917) is a Gram-negative, rod-shaped, facultative anaerobic bacterium |
| Properties: | - |
| Further information: | The strain was isolated from the Flemish drinking water distribution network and was made ampicillin resistant by serial selections on LB agar with increasing concentrations ampicillin. GFP-labelling was performed using triparental mating (donor = Escherichia coli DH5α pME6012 containing the plasmid ptac-gfp; helper = Escherichia coli HB101 containing the plasmid pRK2013 needed for conjugation; acceptor = Serratia fonticola). GFP-labelled coliforms were selected for resistance to tetracycline and ampicillin and screened for GFP fluorescence. |
| Original reference: | Waegenaar et al., bioRxiv (2024) [DOI: 10.1101/2023.09.21.558492] |
| Restricted use: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
|
Refer in your Materials and Methods: |
Serratia fonticola GFP (LMBP 13927) is available at BCCM/GeneCorner. It was deposited by F. Waegenaar and Prof. Dr N. Boon and was published in Waegenaar et al., 2024. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.